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I tested thé quality using Nanódrop and got thé following: 5flies gDNA concentration is 824.1ngul, A260280 is 2.14, and A260230 is 2.06 (I did not use RNase in the extraction protocol) 1fly gNDA concentration is 241.8ngul, A260280 is 2.11, and A260230 is 1.90 (I did not use RNase in the extraction protocol). 2. I ran PCR at 45, 50, 60 degrees.I was wondering if doing this step can cause complications for protein extractions or even imaging the dissected brains using confocal microscopy Thanks in advance for anyones time in answering this.
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